simian virus40 t-antigen cell immortalization kit Search Results


simian virus 40 sv40 t antigen  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology simian virus 40 sv40 t antigen
Simian Virus 40 Sv40 T Antigen, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simian+virus40+t-antigen+cell+immortalization+kit/SV40/pmc00298753-85-13-20
Average 93 stars, based on 1 article reviews
simian virus 40 sv40 t antigen - by Bioz Stars, 2026-09
93/100 stars
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quikchange site-directed mutagenesis kit  (Agilent technologies)
93
Agilent technologies quikchange site-directed mutagenesis kit
Quikchange Site Directed Mutagenesis Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simian+virus40+t-antigen+cell+immortalization+kit/R-Phycoerythrin+Conjugation+Kit/pmc04542342-151-3-5
Average 93 stars, based on 1 article reviews
quikchange site-directed mutagenesis kit - by Bioz Stars, 2026-09
93/100 stars
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gs28 (golgi sampler kit  (Becton Dickinson)
90
Becton Dickinson gs28 (golgi sampler kit
Immunolocalization of Menkes ATPase in Atox1-deficient cells. (A) Immunoblot analysis of Menkes protein in mouse fibroblasts. Cell lysate (100 μg) was separated by 4–20% SDS/PAGE and probed with Menkes antisera (1:2,000) generated against the C terminus of the Menkes ATPase, followed by chemiluminescent detection. Less than 2% of Menkes protein was detected in 802-1 cells compared with wild-type 802-5 cells. (B) Double label indirect immunofluorescence localization (×63) in 802-5 and 802-1 cells grown in basal media, fixed, and stained with <t>GS28</t> (α-GS28, Alexa 488) and Menkes (α-Mnk, Alexa 546) antibodies, and analyzed by using epifluorescence microscopy. Images of Menkes and GS28 <t>Golgi</t> marker are merged to depict overlapping regions. (Scale bar, 50 μm.) (C and D) Immortalized Atox1+/+ and Atox1−/− cell lines were grown in basal media (C) or in the presence of 200 μM BCS (D) and processed for indirect immunofluorescence studies as described above by using epifluorescence microscopy. (Scale bar, 50 μm.)
Gs28 (Golgi Sampler Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simian+virus40+t-antigen+cell+immortalization+kit/mouse+anti+gs28/pmc00298753-41-25-28
Average 90 stars, based on 1 article reviews
gs28 (golgi sampler kit - by Bioz Stars, 2026-09
90/100 stars
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simian virus 40 large t antigen gene plasmid tag-ltr-puc18 sense no. 4  (JCRB Cell Bank)
90
JCRB Cell Bank simian virus 40 large t antigen gene plasmid tag-ltr-puc18 sense no. 4
Immunolocalization of Menkes ATPase in Atox1-deficient cells. (A) Immunoblot analysis of Menkes protein in mouse fibroblasts. Cell lysate (100 μg) was separated by 4–20% SDS/PAGE and probed with Menkes antisera (1:2,000) generated against the C terminus of the Menkes ATPase, followed by chemiluminescent detection. Less than 2% of Menkes protein was detected in 802-1 cells compared with wild-type 802-5 cells. (B) Double label indirect immunofluorescence localization (×63) in 802-5 and 802-1 cells grown in basal media, fixed, and stained with <t>GS28</t> (α-GS28, Alexa 488) and Menkes (α-Mnk, Alexa 546) antibodies, and analyzed by using epifluorescence microscopy. Images of Menkes and GS28 <t>Golgi</t> marker are merged to depict overlapping regions. (Scale bar, 50 μm.) (C and D) Immortalized Atox1+/+ and Atox1−/− cell lines were grown in basal media (C) or in the presence of 200 μM BCS (D) and processed for indirect immunofluorescence studies as described above by using epifluorescence microscopy. (Scale bar, 50 μm.)
Simian Virus 40 Large T Antigen Gene Plasmid Tag Ltr Puc18 Sense No. 4, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simian+virus40+t-antigen+cell+immortalization+kit/sv40st+gene/pm19604164-27-11-24
Average 90 stars, based on 1 article reviews
simian virus 40 large t antigen gene plasmid tag-ltr-puc18 sense no. 4 - by Bioz Stars, 2026-09
90/100 stars
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calphos mammalian transfection kit  (Becton Dickinson)
90
Becton Dickinson calphos mammalian transfection kit
Immunolocalization of Menkes ATPase in Atox1-deficient cells. (A) Immunoblot analysis of Menkes protein in mouse fibroblasts. Cell lysate (100 μg) was separated by 4–20% SDS/PAGE and probed with Menkes antisera (1:2,000) generated against the C terminus of the Menkes ATPase, followed by chemiluminescent detection. Less than 2% of Menkes protein was detected in 802-1 cells compared with wild-type 802-5 cells. (B) Double label indirect immunofluorescence localization (×63) in 802-5 and 802-1 cells grown in basal media, fixed, and stained with <t>GS28</t> (α-GS28, Alexa 488) and Menkes (α-Mnk, Alexa 546) antibodies, and analyzed by using epifluorescence microscopy. Images of Menkes and GS28 <t>Golgi</t> marker are merged to depict overlapping regions. (Scale bar, 50 μm.) (C and D) Immortalized Atox1+/+ and Atox1−/− cell lines were grown in basal media (C) or in the presence of 200 μM BCS (D) and processed for indirect immunofluorescence studies as described above by using epifluorescence microscopy. (Scale bar, 50 μm.)
Calphos Mammalian Transfection Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simian+virus40+t-antigen+cell+immortalization+kit/calphos+mammalian+transfection+kit/pm19604164-27-53-59
Average 90 stars, based on 1 article reviews
calphos mammalian transfection kit - by Bioz Stars, 2026-09
90/100 stars
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qiafiltertm plasmid maxi kit  (Qiagen)
96
Qiagen qiafiltertm plasmid maxi kit
Immunolocalization of Menkes ATPase in Atox1-deficient cells. (A) Immunoblot analysis of Menkes protein in mouse fibroblasts. Cell lysate (100 μg) was separated by 4–20% SDS/PAGE and probed with Menkes antisera (1:2,000) generated against the C terminus of the Menkes ATPase, followed by chemiluminescent detection. Less than 2% of Menkes protein was detected in 802-1 cells compared with wild-type 802-5 cells. (B) Double label indirect immunofluorescence localization (×63) in 802-5 and 802-1 cells grown in basal media, fixed, and stained with <t>GS28</t> (α-GS28, Alexa 488) and Menkes (α-Mnk, Alexa 546) antibodies, and analyzed by using epifluorescence microscopy. Images of Menkes and GS28 <t>Golgi</t> marker are merged to depict overlapping regions. (Scale bar, 50 μm.) (C and D) Immortalized Atox1+/+ and Atox1−/− cell lines were grown in basal media (C) or in the presence of 200 μM BCS (D) and processed for indirect immunofluorescence studies as described above by using epifluorescence microscopy. (Scale bar, 50 μm.)
Qiafiltertm Plasmid Maxi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simian+virus40+t-antigen+cell+immortalization+kit/QIAfilter+Plasmid+Maxi+Kit/pmc03568212-122-52-51
Average 96 stars, based on 1 article reviews
qiafiltertm plasmid maxi kit - by Bioz Stars, 2026-09
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pact2 ( 22 )  (Becton Dickinson)
90
Becton Dickinson pact2 ( 22 )
Immunolocalization of Menkes ATPase in Atox1-deficient cells. (A) Immunoblot analysis of Menkes protein in mouse fibroblasts. Cell lysate (100 μg) was separated by 4–20% SDS/PAGE and probed with Menkes antisera (1:2,000) generated against the C terminus of the Menkes ATPase, followed by chemiluminescent detection. Less than 2% of Menkes protein was detected in 802-1 cells compared with wild-type 802-5 cells. (B) Double label indirect immunofluorescence localization (×63) in 802-5 and 802-1 cells grown in basal media, fixed, and stained with <t>GS28</t> (α-GS28, Alexa 488) and Menkes (α-Mnk, Alexa 546) antibodies, and analyzed by using epifluorescence microscopy. Images of Menkes and GS28 <t>Golgi</t> marker are merged to depict overlapping regions. (Scale bar, 50 μm.) (C and D) Immortalized Atox1+/+ and Atox1−/− cell lines were grown in basal media (C) or in the presence of 200 μM BCS (D) and processed for indirect immunofluorescence studies as described above by using epifluorescence microscopy. (Scale bar, 50 μm.)
Pact2 ( 22 ), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simian+virus40+t-antigen+cell+immortalization+kit/pact2/pmc01214206-166-27-18
Average 90 stars, based on 1 article reviews
pact2 ( 22 ) - by Bioz Stars, 2026-09
90/100 stars
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mouse anti sv40 large  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology mouse anti sv40 large
Immunolocalization of Menkes ATPase in Atox1-deficient cells. (A) Immunoblot analysis of Menkes protein in mouse fibroblasts. Cell lysate (100 μg) was separated by 4–20% SDS/PAGE and probed with Menkes antisera (1:2,000) generated against the C terminus of the Menkes ATPase, followed by chemiluminescent detection. Less than 2% of Menkes protein was detected in 802-1 cells compared with wild-type 802-5 cells. (B) Double label indirect immunofluorescence localization (×63) in 802-5 and 802-1 cells grown in basal media, fixed, and stained with <t>GS28</t> (α-GS28, Alexa 488) and Menkes (α-Mnk, Alexa 546) antibodies, and analyzed by using epifluorescence microscopy. Images of Menkes and GS28 <t>Golgi</t> marker are merged to depict overlapping regions. (Scale bar, 50 μm.) (C and D) Immortalized Atox1+/+ and Atox1−/− cell lines were grown in basal media (C) or in the presence of 200 μM BCS (D) and processed for indirect immunofluorescence studies as described above by using epifluorescence microscopy. (Scale bar, 50 μm.)
Mouse Anti Sv40 Large, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simian+virus40+t-antigen+cell+immortalization+kit/SV40+T+Ag+Antibody/pmc04139384-51-4-12
Average 96 stars, based on 1 article reviews
mouse anti sv40 large - by Bioz Stars, 2026-09
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Image Search Results


Immunolocalization of Menkes ATPase in Atox1-deficient cells. (A) Immunoblot analysis of Menkes protein in mouse fibroblasts. Cell lysate (100 μg) was separated by 4–20% SDS/PAGE and probed with Menkes antisera (1:2,000) generated against the C terminus of the Menkes ATPase, followed by chemiluminescent detection. Less than 2% of Menkes protein was detected in 802-1 cells compared with wild-type 802-5 cells. (B) Double label indirect immunofluorescence localization (×63) in 802-5 and 802-1 cells grown in basal media, fixed, and stained with GS28 (α-GS28, Alexa 488) and Menkes (α-Mnk, Alexa 546) antibodies, and analyzed by using epifluorescence microscopy. Images of Menkes and GS28 Golgi marker are merged to depict overlapping regions. (Scale bar, 50 μm.) (C and D) Immortalized Atox1+/+ and Atox1−/− cell lines were grown in basal media (C) or in the presence of 200 μM BCS (D) and processed for indirect immunofluorescence studies as described above by using epifluorescence microscopy. (Scale bar, 50 μm.)

Journal:

Article Title: Essential role for Atox1 in the copper-mediated intracellular trafficking of the Menkes ATPase

doi: 10.1073/pnas.0336230100

Figure Lengend Snippet: Immunolocalization of Menkes ATPase in Atox1-deficient cells. (A) Immunoblot analysis of Menkes protein in mouse fibroblasts. Cell lysate (100 μg) was separated by 4–20% SDS/PAGE and probed with Menkes antisera (1:2,000) generated against the C terminus of the Menkes ATPase, followed by chemiluminescent detection. Less than 2% of Menkes protein was detected in 802-1 cells compared with wild-type 802-5 cells. (B) Double label indirect immunofluorescence localization (×63) in 802-5 and 802-1 cells grown in basal media, fixed, and stained with GS28 (α-GS28, Alexa 488) and Menkes (α-Mnk, Alexa 546) antibodies, and analyzed by using epifluorescence microscopy. Images of Menkes and GS28 Golgi marker are merged to depict overlapping regions. (Scale bar, 50 μm.) (C and D) Immortalized Atox1+/+ and Atox1−/− cell lines were grown in basal media (C) or in the presence of 200 μM BCS (D) and processed for indirect immunofluorescence studies as described above by using epifluorescence microscopy. (Scale bar, 50 μm.)

Article Snippet: Rabbit polyclonal antibody to SOD1 (Stressgen Biotechnologies, Victoria, Canada), mouse monoclonal antibodies to simian virus 40 (SV40) T antigen (Pab101):sc147 (Santa Cruz Biotechnology) and GS28 (Golgi Sampler kit; BD Biosciences) were used according to the manufacturer's recommendations.

Techniques: Western Blot, SDS Page, Generated, Immunofluorescence, Staining, Epifluorescence Microscopy, Marker

Characterization of copper-mediated trafficking of Menkes protein. (A) Immortalized Atox1+/+ and Atox1−/− cells were grown on coverslips in the presence of 200 μM BCS for 48 h, followed by the addition of 0.5 or 10 μM CuCl2 for 1 h to the culture media, and processed for double-label immunofluorescence by using Menkes and GS28 antibodies for analysis with confocal microscopy. Images of Menkes and GS28 are merged to depict overlapping regions. (Scale bar, 50 μm.) (B) Quantitative analysis of Menkes signal in the Golgi was measured in immortalized Atox1+/+ cells by using double-label immunofluorescence studies as described in Materials and Methods. Cells were grown in the presence of 200 μM BCS followed by copper treatment for 3 h, and processed for immunostaining. Overlapping signal intensities were analyzed and quantitated by using a laser scanning confocal Olympus microscope and FLUOVIEW FV500 Version 3.3 program. Each data point represents the mean ± SEM from four separate experiments with a minimum of seven scans per experiment per data point. Each scan is comprised of 15–18 cells or at least 105 cells. (Inset) The same analysis plotted onto a logarithmic x axis (μM) for clarity.

Journal:

Article Title: Essential role for Atox1 in the copper-mediated intracellular trafficking of the Menkes ATPase

doi: 10.1073/pnas.0336230100

Figure Lengend Snippet: Characterization of copper-mediated trafficking of Menkes protein. (A) Immortalized Atox1+/+ and Atox1−/− cells were grown on coverslips in the presence of 200 μM BCS for 48 h, followed by the addition of 0.5 or 10 μM CuCl2 for 1 h to the culture media, and processed for double-label immunofluorescence by using Menkes and GS28 antibodies for analysis with confocal microscopy. Images of Menkes and GS28 are merged to depict overlapping regions. (Scale bar, 50 μm.) (B) Quantitative analysis of Menkes signal in the Golgi was measured in immortalized Atox1+/+ cells by using double-label immunofluorescence studies as described in Materials and Methods. Cells were grown in the presence of 200 μM BCS followed by copper treatment for 3 h, and processed for immunostaining. Overlapping signal intensities were analyzed and quantitated by using a laser scanning confocal Olympus microscope and FLUOVIEW FV500 Version 3.3 program. Each data point represents the mean ± SEM from four separate experiments with a minimum of seven scans per experiment per data point. Each scan is comprised of 15–18 cells or at least 105 cells. (Inset) The same analysis plotted onto a logarithmic x axis (μM) for clarity.

Article Snippet: Rabbit polyclonal antibody to SOD1 (Stressgen Biotechnologies, Victoria, Canada), mouse monoclonal antibodies to simian virus 40 (SV40) T antigen (Pab101):sc147 (Santa Cruz Biotechnology) and GS28 (Golgi Sampler kit; BD Biosciences) were used according to the manufacturer's recommendations.

Techniques: Immunofluorescence, Confocal Microscopy, Immunostaining, Microscopy